To study the effect of x-ray radiotherapy on vascular smooth muscle cells (VSMCs) and elucidate the mechanisms in preventing neointimal hyperplasia of prosthetic vascular grafts.
In model I, twelve mongrel dogs underwent revascularization with prosthetic grafts and half the dogs underwent irradiation of the grafts at 28 Gy. In model II, human VSMCs (hVSMCs) were maintained and divided into six groups to which external radiation was applied at six different doses: 0 Gy, 2 Gy, 8 Gy, 16 Gy, 24 Gy and 30 Gy. In both models, specimens were harvested and examined by using morphological, immunological, cellular and molecular methods.
After irradiation, the neointima thickness was significantly lower in irradiated groups (p≤0.01). The radiotherapy could up-regulate p27kip1, and down-regulate proliferating cell nuclear antigen (PCNA) and S phase kinase associated protein 2 (Skp2). X-ray irradiation inhibits the proliferation of hVSMCs via acting on G1/S phase of cell cycle. The apoptosis of hVSMCs increased significantly with dose and time. The expression of PCNA and Skp2 were decreased after a first increasing trend with dose, but had a significant negative correlation with time. The expression of p27kip1 had a significant positive correlation with dose and time.
Postoperative external fractionated irradiation after prosthetic vessel replacement of the abdominal aorta suppressed the development of hyperplasia in the graft neointima in the short term. There was a prominent time- and dose-dependent inhibition of VSMC proliferation by radiation when it was administered.
Post author correction
Article Type: ORIGINAL RESEARCH ARTICLE
Article Subject: Interventional radiology
AuthorsLiqin Yuan, Chang Shu, Xiao Zhou, Jiehua Li, Lunchang Wang, Xin Li, Xiang Xiong, Lun Li
- • Accepted on 30/07/2017
- • Available online on 29/11/2017
This article is available as full text PDF.
- Yuan, Liqin [PubMed] [Google Scholar] 1
- Shu, Chang [PubMed] [Google Scholar] 2, * Corresponding Author (email@example.com)
- Zhou, Xiao [PubMed] [Google Scholar] 3
- Li, Jiehua [PubMed] [Google Scholar] 2
- Wang, Lunchang [PubMed] [Google Scholar] 2
- Li, Xin [PubMed] [Google Scholar] 2
- Xiong, Xiang [PubMed] [Google Scholar] 1
- Li, Lun [PubMed] [Google Scholar] 1
Department of General Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan - China
Department of Vascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan - China
Department of Head and Neck Surgery, The affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan - China